E.coli O157:H7 PCR ngwa nchọpụta

Nkọwa dị mkpirikpi:

Escherichia coli O157:H7 (E.coli O157:H7) bụ gram-adịghị mma nje bacteria nke genus Enterobacteriaceae, nke na-emepụta nnukwu ego nke Vero toxin.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

E.coli O157:H7 PCR ngwa nchọpụta (Lyophilized)

Nha

48 ule / ngwa, 50 ule / ngwa

Ezubere iji

Escherichia coli O157:H7 (E.coli O157:H7) bụ gram-adịghị mma nje bacteria nke genus Enterobacteriaceae, nke na-emepụta nnukwu ego nke Vero toxin.N'ụlọ ọgwụ, ọ na-emekarị na mberede na oke mgbu afọ na afọ ọsịsa mmiri, afọ ọsịsa hemorrhagic na-esote ụbọchị ole na ole ka e mesịrị, nke nwere ike ịkpata ahụ ọkụ ma ọ bụ enweghị ahụ ọkụ, na ọnwụ n'ọnọdụ ndị siri ike.Nke a kit bụ adabara qualitative nchọpụta nke Escherichia coli O157: H7 na nri, mmiri samples, feces, vomit, bacterium-eme ka mmiri mmiri na ndị ọzọ samples iji ụkpụrụ nke ezigbo oge PCR.The kit bụ ihe niile-njikere PCR usoro ( Lyophilized), nke nwere enzyme amplification DNA, ihe nchekwa mmeghachi omume, ihe izizi na nyocha achọrọ maka nchọpụta PCR fluorescent.

Ọdịnaya ngwaahịa

Ngwa ngwugwu nkọwapụta Ngwakọta
E.coli O157:H7 PCR Ngwakọta 1 × karama (Lyophilized ntụ ntụ)  50Nnwale dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 nke ọma warara tube(Lyophilized) 48Nnwale
Njikwa ziri ezi 1 * 0.2ml tube (lyophilized)  10Nnwale

Plasmid nwere E.coli O157:H7 kpọmkwem iberibe

Ngwọta mgbaze 1.5 ml nke Cryotube 500ul /
Njikwa adịghị mma 1.5 ml nke Cryotube 200ul 0.9% NaCl

Nchekwa & Ndụ shelf

(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.

(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.

(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.

(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.

Ngwa ngwa

GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.

Eserese ọrụ

a) Ụdị karama:

1

b) 8 ụdị tube nke ọma:

2

Pcr Amplification

AkwadoroỊtọ ntọala

Nzọụkwụ okirikiri Okpomọkụ (℃) Oge Ọwa fluorescence
1 1 95 Nkeji 2  
2 40 95 5s  
60 10s Anakọta FAM fluorescence

* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.

Nsonaazụ ule ntụgharị asụsụ

Ọwa

Nkọwa nke nsonaazụ

Ọwa FAM

Ct≤35

E.coli O157:H7 Ezi

Undet

E.coli O157:H7 adịghị mma

35

Nsonaazụ enyo enyo, nwale*

* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.


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