Pseudorabies virus (gB) ihe nchọpụta PCR

Nkọwa nkenke:

Ngwa a na-eji usoro PCR fluorescent oge iji chọpụta RNA nke nje Pseudorabies (gB gene) (PRV) na arịa ọrịa anụ ahụ dịka tonsils, lymph nodes na splin na mmiri ọrịa ihe dịka ogwu na ọbara ezì.


Product Ihe

Ngwaahịa

Aha ngwaahịa

Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Nha

Ntughari 48 / kit, 50tests / kit

Bu n'obi Jiri

Ngwa a na-eji usoro PCR fluorescent oge iji chọpụta RNA nke nje Pseudorabies (gB gene) (PRV) na arịa ọrịa anụ ahụ dịka tonsils, lymph nodes na splin na mmiri ọrịa ihe dịka ogwu na ọbara ezì. Okwesiri maka nchoputa, nchoputa na nyocha oria ojoo nke oria Pseudorabies (gB gene). Ihe ngwugwu a bu ihe omuma PCR nile (Lyophilized), nke nwere enzyme nke DNA na-emewanye ihe, ihe nkpuchi mmeghachi omume, ihe ntanye anya na nyocha a choro maka nchoputa RT-PCR.

Ọdịnaya Ngwaahịa

Akụkụ Ngwugwu nkọwapụta Ngwa
Ngwakọta PCV PCR 1 × karama (Lyophilized ntụ ntụ)  50Test dNTPs, MgCl2, Akwụkwọ, Nyocha, Taq DNA polymerase
6 × 0.2ml 8 tube warara nke ọma(Lyophilized) 48Gwa
Njikwa Ọma 1 * 0.2ml tube (lyophilized)  10 Ule

Plasmid ma ọ bụ Pseudovirus nwere PRV (gB) ụfọdụ iberibe

Mgbasa ngwọta 1.5 ml Cryotube 500UL /
Nchịkwa na-adịghị mma 1.5 ml Cryotube 200UL 0,9% NaCl

Nchekwa & adị ndụ

(1) Enwere ike ibuga kit ahụ na ọnọdụ okpomọkụ.

(2) Ndụ ndụ bụ ọnwa 18 na -20 ℃ na ọnwa 12 na 2 ℃ ~ 30 ℃.

(3) Hụ akara dị na kit maka ụbọchị mmepụta na ụbọchị mmebi.

(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa ya na ugboro ugboro ifriizi -gha ga-erughị 4 ugboro.

Ngwaọrụ

GENECHECKER UF-150, UF-300 ezigbo oge ihe eji arụ ọrụ PCR.

Ihe osise

a) Kalama ụdị:

1

b) 8 nke di nma tube:

2

Mmezi PCr

Na-atụ aro Ntọala

Nzọụkwụ

Okirikiri

Okpomọkụ (℃)

Oge

Fluorescence ọwa

1

1

95

2min

/

2

40

95

5s

/

60

10s

Chịkọta FAM fluorescence

* Mara: A ga-anakọta akara nke ọwa FAM fluorescence na 60 ℃.

Resultskọwa Ule Nsonaazụ

Ọwa

Nkọwa nke nsonaazụ

FAM Ọwa

CT≤35

PRV (gB) Nke oma

Mechie

PRV (gB) Ihe ojoo

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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