中文Pseudorabies virus (gB) PCR nchọpụta ngwa
Aha ngwaahịa
Pseudorabies nje (gB) PCR Nchọpụta ngwa (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ngwa a na-eji usoro PCR fluorescent ezigbo oge iji chọpụta RNA nke nje Pseudorabies (gB gene) (PRV) n'ime ihe ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na ihe ọrịa mmiri mmiri dị ka ọgwụ mgbochi na ọbara ezi.Ọ dabara maka nchọpụta, nchọpụta na nchọpụta ọrịa ọrịa Pseudorabies virus (gB gene).Ngwa ahụ bụ sistemu PCR dị njikere (Lyophilized), nke nwere enzyme amplification DNA, ihe nchekwa mmeghachi omume, ihe izizi na nyocha nke achọrọ maka nchọpụta fluorescent RT-PCR.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta PRV PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus nwere iberibe PRV(gB) kpọmkwem |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 95 | Nkeji 2 | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | PRV(gB) dị mma |
| Undet | PRV(gB) adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
