中文Porcine Circovirus ụdị 2 PCR nchọpụta ngwa
Aha ngwaahịa
Porcine circovirus ụdị 2 PCR Detection Kit (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ngwa a na-eji ezigbo oge fluorescent PCR usoro iji chọpụta RNA nke Porcine circovirus ụdị 2 (PCV2) na ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na mmiri mmiri ihe dị ka ọgwụ mgbochi na ọbara nke.Ọ dị mma maka nchọpụta, nchọpụta na nchọpụta ọrịa ọrịa nke Porcine circovirus ụdị 2. Ngwa ahụ bụ PCR SYSTEM (Lyophilized), nke nwere DNA amplification enzyme, mmeghachi omume mmeghachi omume, kpọmkwem primers na nyocha achọrọ maka nchọpụta PCR fluorescent. .
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta PCV2 PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus PCV2 nwere obere iberibe |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 95 | Nkeji 2 | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | Vibrio Parahaemolyticus dị mma |
| Undet | Vibrio Parahaemolyticus adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
