中文Ihe nchọta RT-PCR ọrịa ụkwụ na ọnụ
Aha ngwaahịa
Nje virus nke ụkwụ na ọnụ RT-PCR Nchọpụta Kit (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ngwa a na-eji ezigbo fluorescent RT-PCR usoro iji chọpụta RNA nke ọrịa ụkwụ na ọnụ (CSFV) n'ime ihe ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na ihe ọrịa mmiri mmiri dị ka ọgwụ mgbochi na ọbara ezi.Ọ dabara maka nchọpụta, nchọpụta na nchọpụta ọrịa na-efe efe nke ọrịa ụkwụ na ọnụ.Ngwa ahụ bụ usoro PCR dị njikere niile (Lyophilized), nke nwere enzyme amplification DNA, reverse transcriptase, ihe nchekwa mmeghachi omume, ihe mbido na nyocha achọrọ maka nchọpụta PCR fluorescent.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta FMD PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid nwere iberibe FMD kpọmkwem |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
AkwadoroỊtọ ntọala
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | Nkeji 2 | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | FMD dị mma |
| Undet | FMD adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
