Nje virus nke ịmụ nwa na akụkụ iku ume nke RT-PCR

Nkọwa dị mkpirikpi:

Ngwa a na-eji ezigbo fluorescent RT-PCR usoro iji chọpụta RNA nke Porcine reproductive and respiratory syndrome virus nucleic acid detection kit (PRRSV) na ihe ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na mmiri ọrịa ihe dị ka ọgwụ mgbochi na ọbara. nke ezi .


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

Ihe na-achọpụta ihe nrịbama nke anụ ahụ na ọrịa iku ume RT-PCR (Lyophilized)

Nha

48 ule / ngwa, 50 ule / ngwa

Ezubere iji

Ngwa a na-eji ezigbo fluorescent RT-PCR usoro iji chọpụta RNA nke Porcine reproductive and respiratory syndrome virus nucleic acid detection kit (PRRSV) na ihe ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na mmiri ọrịa ihe dị ka ọgwụ mgbochi na ọbara. nke ezi .Ọ dabara maka nchọpụta, nchoputa na nchọpụta ọrịa gbasara nje Porcine Blue Ear.Ngwa ahụ bụ sistemu PCR dị njikere (Lyophilized), nke nwere reverse transcriptase, enzyme amplification enzyme, ihe nchekwa mmeghachi omume, ihe izizi na nyocha achọrọ maka nchọpụta fluorescent RT-PCR.

Ọdịnaya ngwaahịa

Ngwa ngwugwu nkọwapụta Ngwakọta
Ngwakọta nke PBEV PCR 1 × karama (Lyophilized ntụ ntụ)  50Nnwale dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 nke ọma warara tube(Lyophilized) 48Nnwale
Njikwa ziri ezi 1 * 0.2ml tube (lyophilized)  10Nnwale

Plasmid ma ọ bụ Pseudovirus nwere iberibe PRRSV kpọmkwem

Ngwọta mgbaze 1.5 ml nke Cryotube 500ul /
Njikwa adịghị mma 1.5 ml nke Cryotube 200ul 0.9% NaCl

Nchekwa & Ndụ shelf

(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.

(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.

(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.

(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.

Ngwa ngwa

GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.

Eserese ọrụ

a) Ụdị karama:

1

b) 8 ụdị tube nke ọma:

2

Pcr Amplification

Ntọala akwadoro

Nzọụkwụ

okirikiri

Okpomọkụ (℃)

Oge

Ọwa fluorescence

1

1

48

8 min

/

2

1

95

Nkeji 2

/

3

40

95

5s

/

60

10s

Anakọta FAM fluorescence

* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.

Nsonaazụ ule ntụgharị asụsụ

Ọwa

Nkọwa nke nsonaazụ

Ọwa FAM

Ct≤35

Vibrio Parahaemolyticus dị mma

Undet

Vibrio Parahaemolyticus adịghị mma

35

Nsonaazụ enyo enyo, nwale*

* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.


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