E.coli O157: H7 PCR na-achọpụta ihe

Nkọwa nkenke:

Escherichia coli O157: H7 (E.coli O157: H7) bụ nje na-adịghị na gram na-adịghị mma nke ụdị Genero Enterobacteriaceae, nke na-ewepụta oke nke toxin Vero.


Product Ihe

Ngwaahịa

Aha ngwaahịa

E.coli O157: H7 PCR nchọpụta kit (Lyophilized)

Nha

Ntughari 48 / kit, 50tests / kit

Bu n'obi Jiri

Escherichia coli O157: H7 (E.coli O157: H7) bụ nje na-adịghị na gram na-adịghị mma nke ụdị Genero Enterobacteriaceae, nke na-ewepụta oke nke toxin Vero. N'ihe gbasara ahụike, ọ na-abụkarị na mberede na nnukwu ihe mgbu afọ na afọ ọsịsa mmiri, na-eso afọ ọsịsa nke ọbara ọgbụgba ụbọchị ole na ole gachara, nke nwere ike ibute ahụ ọkụ ma ọ bụ enweghị ahụ ọkụ, na ọnwụ na oke nsogbu. Ngwa a kwesịrị ekwesị maka nchọpụta qualitative nke Escherichia coli O157: H7 na nri, mmiri mmiri, nsị, ọgbụgbọ, mmiri ọgwụ na-eme ka nje na ihe ndị ọzọ na-eji ụkpụrụ nke PCR oge. Lyophilized), nke nwere enzyme nke mmụba DNA, ihe nchekwa mmeghachi omume, mpempe akwụkwọ ụfọdụ na nyocha chọrọ maka nchọpụta PCR fluorescent ..

Ọdịnaya Ngwaahịa

Akụkụ Ngwugwu nkọwapụta Ngwa
E.coli O157: H7 PCR Ngwakọta 1 × karama (Lyophilized ntụ ntụ)  50Test dNTPs, MgCl2, Akwụkwọ, Nyocha, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 tube warara nke ọma(Lyophilized) 48Gwa
Njikwa Ọma 1 * 0.2ml tube (lyophilized)  10 Ule

Plasmid nwere E.coli O157: H7 iberibe ụfọdụ

Mgbasa ngwọta 1.5 ml Cryotube 500UL /
Nchịkwa na-adịghị mma 1.5 ml Cryotube 200UL 0,9% NaCl

Nchekwa & adị ndụ

(1) Enwere ike ibuga kit ahụ na ọnọdụ okpomọkụ.

(2) Ndụ ndụ bụ ọnwa 18 na -20 ℃ na ọnwa 12 na 2 ℃ ~ 30 ℃.

(3) Hụ akara dị na kit maka ụbọchị mmepụta na ụbọchị mmebi.

(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa ya na ugboro ugboro ifriizi -gha ga-erughị 4 ugboro.

Ngwaọrụ

GENECHECKER UF-150, UF-300 ezigbo oge ihe eji arụ ọrụ PCR.

Ihe osise

a) Kalama ụdị:

1

b) 8 nke di nma tube:

2

Mmezi PCr

Na-atụ aro Ntọala

Nzọụkwụ     Okirikiri Okpomọkụ (℃)   Oge Fluorescence ọwa
1 1 95 2min  
2 40 95 5s  
60 10s Chịkọta FAM fluorescence

* Mara: A ga-anakọta akara nke ọwa FAM fluorescence na 60 ℃.

Resultskọwa Ule Nsonaazụ

Ọwa

Nkọwa nke nsonaazụ

FAM Ọwa

CT≤35

E.coli O157: H7 Positive

Mechie

E.coli O157: H7 Njọ

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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