中文E.coli O157:H7 PCR ngwa nchọpụta
Aha ngwaahịa
E.coli O157:H7 PCR ngwa nchọpụta (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Escherichia coli O157:H7 (E.coli O157:H7) bụ gram-adịghị mma nje bacteria nke genus Enterobacteriaceae, nke na-emepụta nnukwu ego nke Vero toxin.N'ụlọ ọgwụ, ọ na-emekarị na mberede na oke mgbu afọ na afọ ọsịsa mmiri, afọ ọsịsa hemorrhagic na-esote ụbọchị ole na ole ka e mesịrị, nke nwere ike ịkpata ahụ ọkụ ma ọ bụ enweghị ahụ ọkụ, na ọnwụ n'ọnọdụ ndị siri ike.Nke a kit bụ adabara qualitative nchọpụta nke Escherichia coli O157: H7 na nri, mmiri samples, feces, vomit, bacterium-eme ka mmiri mmiri na ndị ọzọ samples iji ụkpụrụ nke ezigbo oge PCR.The kit bụ ihe niile-njikere PCR usoro ( Lyophilized), nke nwere enzyme amplification DNA, ihe nchekwa mmeghachi omume, ihe izizi na nyocha achọrọ maka nchọpụta PCR fluorescent.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| E.coli O157:H7 PCR Ngwakọta | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid nwere E.coli O157:H7 kpọmkwem iberibe |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
AkwadoroỊtọ ntọala
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 95 | Nkeji 2 | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | E.coli O157:H7 Ezi |
| Undet | E.coli O157:H7 adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
