Ngwa nchọpụta PCR Shigella

Nkọwa dị mkpirikpi:

Shigella bụ ụdị gram-negative brevis bacilli, nke bụ nke nje nje eriri afọ, na ihe na-ebutekarị ọrịa ọnyụnyụ ọbara mmadụ.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

Ngwa ihe nchọpụta Shigella PCR (lyophilized)

Nha

48 ule / ngwa, 50 ule / ngwa

Ezubere iji

Shigella bụ ụdị gram-negative brevis bacilli, nke bụ nke nje nje eriri afọ, na ihe na-ebutekarị ọrịa ọnyụnyụ ọbara mmadụ.Ngwa a na-eji ụkpụrụ nke PCR fluorescent ezigbo oge, nke dabara maka mkpebi Shigella na nri, ihe nlele mmiri, nsị, ọgbụgbọ, na mmiri ịba ụba.

Ọdịnaya ngwaahịa

Ngwa ngwugwu nkọwapụta Ngwakọta
Ngwakọta PCR 1 × karama (Lyophilized ntụ ntụ)  50Nnwale dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase
6 × 0.2ml 8 nke ọma warara tube(Lyophilized) 48Nnwale
Njikwa ziri ezi 1 * 0.2ml tube (lyophilized)  10Nnwale

Plasmid ma ọ bụ Pseudovirus nwere obere iberibe

Ngwọta mgbaze 1.5 ml nke Cryotube 500ul /
Njikwa adịghị mma 1.5 ml nke Cryotube 100uL 0.9% NaCl

Nchekwa & Ndụ shelf

(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.

(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.

(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.

(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.

Ngwa ngwa

GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.

Eserese ọrụ

a) Ụdị karama:

1

b) 8 ụdị tube nke ọma:

2

Pcr Amplification

Ntọala akwadoro

Nzọụkwụ

okirikiri

Okpomọkụ (℃)

Oge

Ọwa fluorescence

1

1

95

Nkeji 2

/

2

40

95

5s

/

60

10s

Anakọta FAM fluorescence

Nsonaazụ ule ntụgharị asụsụ

Ọwa

Nkọwa nke nsonaazụ

Ọwa FAM

Ct≤35

Shigella dị mma

Undet

Shigella Negative

35

Nsonaazụ enyo enyo, nwale*

* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.


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