中文Ihe nchọta Norovirus (GⅠ) RT-PCR
Aha ngwaahịa
Ihe nchọta Norovirus (GⅠ) RT-PCR (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ọ dabara maka ịchọpụta Norovirus (GⅠ) na azụ azụ, inine na mkpụrụ osisi, mmiri, nsị, vomit na ụdị ndị ọzọ.Ekwesịrị ime mmịpụta nucleic acid site na ngwa nucleic acid mmịpụta ma ọ bụ usoro pyrolysis kpọmkwem dị ka ụdị ụdị ụdị dị iche iche si dị. Ngwa ahụ bụ usoro PCR READY NIILE (Lyophilized), nke nwere enzyme amplification DNA, reverse transcriptase, reaction buffer, kpọmkwem primers. yana nyocha achọrọ maka nchọpụta PCR fluorescent.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Norovirus (GⅠ) PCR Mix | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid nwere Norovirus (GⅠ) obere iberibe |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
AkwadoroỊtọ ntọala
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | Nkeji 2 | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | Norovirus (GⅠ) dị mma |
| Undet | Norovirus (GⅠ) adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
