中文Ngwa nyocha Staphylococcus aureus PCR
Aha ngwaahịa
Staphylococcus aureus Ngwa nchọpụta PCR (lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Staphylococcus aureus bụ nke genus Staphylococcus ma bụrụ nje bacteria nwere gram.Ọ bụ microorganism pathogenic na-ebutekarị nri nke nwere ike ịmepụta enterotoxins ma kpatara nsị nri.Ngwa a na-eji ụkpụrụ nke PCR fluorescent dị adị ma dabara adaba maka ịchọpụta Staphylococcus aureus na nri, ihe nlele mmiri, feces, vomit, mmiri ịba ụba na ihe nlele ndị ọzọ.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus nwere obere iberibe |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 100uL | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 95 | Nkeji 2 | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Anakọta FAM fluorescence |
Nsonaazụ ule ntụgharị asụsụ
| Channel | Nkọwa nke nsonaazụ |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Nke dị mma |
| Undet | Staphylococcus aureus Ihe ọjọọ |
| 35<Ct≤40 | Na-enyo enyopụta,nwalee* |
* Ọ bụrụnke Nnwale nsonaazụ nke ọwa FAMhasỌnụ ego Ct ≤40na-egosi ụdị"S”udi mmụba akụkụ, A na-akọwa nsonaazụ ya dị ka ihe dị mma, ma ọ bụghị na ọ dị njọ.
