中文Ngwa ihe nchọpụta Vibrio parahaemolyticus PCR
Aha ngwaahịa
Ngwa nchọpụta Vibrio Parahaemolyticus PCR (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Vibrio Parahemolyticus (nke a makwaara dị ka Halophile Vibrio Parahemolyticus) bụ gram-negative polymorphic bacillus ma ọ bụ Vibrio Parahemolyticus.with nnukwu mmalite, mgbu afọ, vomiting, afọ ọsịsa na mmiri stool dị ka isi ụlọ ọgwụ mgbaàmà.This kit na-eji ụkpụrụ nke ezigbo fluorescent oge. PCR ma dabara adaba maka nchọpụta qualitative nke Vibrio Parahaemolyticus na nri, ihe nlele mmiri, feces, vomit, na mmiri ịba ụba. Ngwa ahụ bụ usoro PCR (Lyophilized), nke nwere DNA amplification enzyme, nzaghachi mmeghachi omume, kpọmkwem primers. yana nyocha achọrọ maka nchọpụta fluorescent RT-PCR.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus nwere obere iberibe |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 100uL | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 95 | Nkeji 2 | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Anakọta FAM fluorescence |
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | Vibrio Parahaemolyticus dị mma |
| Undet | Vibrio Parahaemolyticus adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
