中文Ngwa nchọpụta RT-PCR na-efe efe ọrịa afọ ọsịsa
Aha ngwaahịa
Ọrịa afọ ọsịsa na-efe efe nke porcine RT-PCR Nchọpụta Kit (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ngwa a na-eji ezigbo fluorescent RT-PCR usoro iji chọpụta RNA nke Porcine ọrịa afọ ọsịsa ọrịa (PEDV) na ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na mmiri mmiri ihe dị ka ọgwụ mgbochi na ọbara ezi.Ọ dị mma maka nchọpụta, nchọpụta na nchọpụta ọrịa ọrịa nke ọrịa afọ ọsịsa na-efe efe nke Porcine.Ngwa ahụ bụ sistemu PCR dị njikere (Lyophilized), nke nwere reverse transcriptase, DNA amplification enzyme, buffer mmeghachi omume, primers kpọmkwem na nyocha achọrọ maka nchọpụta fluorescent RT-PCR.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta nke PEDV PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus nwere iberibe PEDV kpọmkwem |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | Nkeji 2 | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | PEDV dị mma |
| Undet | PEDV adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
