中文Nje virus nke ịmụ nwa na akụkụ iku ume nke RT-PCR
Aha ngwaahịa
Ihe na-achọpụta ihe nrịbama nke anụ ahụ na ọrịa iku ume RT-PCR (Lyophilized)
Nha
48 ule / ngwa, 50 ule / ngwa
Ezubere iji
Ngwa a na-eji ezigbo fluorescent RT-PCR usoro iji chọpụta RNA nke Porcine reproductive and respiratory syndrome virus nucleic acid detection kit (PRRSV) na ihe ọrịa anụ ahụ dị ka tonsils, lymph nodes na splin na mmiri ọrịa ihe dị ka ọgwụ mgbochi na ọbara. nke ezi .Ọ dabara maka nchọpụta, nchoputa na nchọpụta ọrịa gbasara nje Porcine Blue Ear.Ngwa ahụ bụ sistemu PCR dị njikere (Lyophilized), nke nwere reverse transcriptase, enzyme amplification enzyme, ihe nchekwa mmeghachi omume, ihe izizi na nyocha achọrọ maka nchọpụta fluorescent RT-PCR.
Ọdịnaya ngwaahịa
| Ngwa | ngwugwu | nkọwapụta | Ngwakọta |
| Ngwakọta nke PBEV PCR | 1 × karama (Lyophilized ntụ ntụ) | 50Nnwale | dNTP, mgCl2, Ndị mbụ, Nyocha, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 nke ọma warara tube(Lyophilized) | 48Nnwale | ||
| Njikwa ziri ezi | 1 * 0.2ml tube (lyophilized) | 10Nnwale | Plasmid ma ọ bụ Pseudovirus nwere iberibe PRRSV kpọmkwem |
| Ngwọta mgbaze | 1.5 ml nke Cryotube | 500ul | / |
| Njikwa adịghị mma | 1.5 ml nke Cryotube | 200ul | 0.9% NaCl |
Nchekwa & Ndụ shelf
(1) Enwere ike ibuga ngwa ahụ na okpomọkụ ụlọ.
(2) The shelf ndụ bụ 18 ọnwa na -20 ℃ na 12 ọnwa na 2 ℃ ~ 30 ℃.
(3) Hụ akara na kit maka ụbọchị mmepụta na ụbọchị njedebe.
(4) The lyophilized ntụ ntụ version reagent kwesịrị ịchekwa na -20 ℃ mgbe mgbasa na ugboro ugboro ifriizi-thaw kwesịrị ihe na-erughị 4 ugboro.
Ngwa ngwa
GENECHECKER UF-150, UF-300 ezigbo oge fluorescence PCR akụrụngwa.
Eserese ọrụ
a) Ụdị karama:
b) 8 ụdị tube nke ọma:
Pcr Amplification
Ntọala akwadoro
| Nzọụkwụ | okirikiri | Okpomọkụ (℃) | Oge | Ọwa fluorescence |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | Nkeji 2 | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Anakọta FAM fluorescence |
* Mara: A ga-anakọta akara ngosi nke ọwa fluorescence FAM na 60 ℃.
Nsonaazụ ule ntụgharị asụsụ
| Ọwa | Nkọwa nke nsonaazụ |
| Ọwa FAM | |
| Ct≤35 | Vibrio Parahaemolyticus dị mma |
| Undet | Vibrio Parahaemolyticus adịghị mma |
| 35 | Nsonaazụ enyo enyo, nwale* |
* Ọ bụrụ na nnwale nyocha nke chanel FAM nwere uru Ct ≤40 wee gosi usoro nkwalite ụdị “S” a na-ahụkarị, a na-atụgharị nsonaazụ ya ka ọ dị mma, ma ọ bụghị na ọ dị njọ.
